Sandwitch ELISA Principle

Sandwich ELISA – Definition, Principle, Steps, Advantages The sandwich ELISA (Enzyme-Linked Immunosorbent Assay) is a widely used technique in immunology and biochemistry to quantify the concentration of specific antigens in a sample. It derives its name from the fact that the antigen of interest is sandwiched between two layers of antibodies, known as the capture antibody and the detection antibody.